Reporter

Part:BBa_K2088008

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-08)


catechol 2,3-dioxygenase gene with native rbs

This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 and its native ribosome binding site. With a promoter before them, this gene can be used to degrade catechol, yeilding bright yellow product 2-hydroxymuconate semialdehyde with an absorbance maximum around 375 nm. As a result, this part can also be utilized as a reporter. As for the gene expression result please visit part BBa_K2088009.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 839


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Categories
Parameters
None